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Lab Manual and Workbook in Microbiology Applications to Patient Care 12th Edition By Josephine Morello

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337
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11.9 MB
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Digital PDF
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The Lab Manual and Workbook in Microbiology: Applications to Patient Care (12th Edition) by Josephine A. Morello, Paul A. Granato, and Verna Morton emphasizes the foundational principles of diagnostic microbiology tailored for allied health and nursing students. [1, 2]
The book is structured around 34 core exercises and advanced diagnostic techniques, categorized into several major operational and clinical topics: [1, 2]
🔬 Basic Techniques, Microscopy & Staining
  • The Microscope: Mechanics and proper handling.
  • Culture Observation: Handling, cultivating, and examining microbial cultures.
  • Staining Methods: Simple stains, hanging drop/wet-mount preparations, Gram stains, and acid-fast stains. [1]
🧫 Isolation and Cultivation Techniques
  • Media Preparation: Understanding types of culture media.
  • Pure Cultures: The streaking technique to isolate colonies and subculture techniques. [1]
🌡️ Physical and Chemical Control of Microorganisms
  • Sterilization & Heat: Mechanisms of moist heat, dry heat, and using the autoclave.
  • Disinfectants: Evaluating chemical agent efficiency.
  • Antimicrobial Susceptibility: Testing methods and evaluating bacterial drug resistance. [1]
🧬 Bacterial Identification & Clinical Diagnostics
  • Advanced Diagnostics & Biochemistry: Covers metabolic biochemical profiles, immunological assays, nucleic acid assays, and MALDI-TOF mass spectrometry. [1]
  • Medical Bacteriology: Explores Gram-positive/negative bacteria, enteric pathogens, and specific specimen processing across various anatomical sites. [1]
  • Specialized Pathogens & Organisms: Includes coverage of anaerobes, mycobacteria, viruses, fungi, and medical parasites. [1]
  • Infection Control: Reviews disease serodiagnosis and modern prevention protocols. [1]

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