Gene Cloning and DNA Analysis: An Introduction 8th Edition By T. A. Brown
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Gene Cloning and DNA Analysis: An Introduction 8th Edition By T. A. Brown

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435
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37.11 MB
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About this ebook
The 8th Edition of Gene Cloning and DNA Analysis: An Introduction by T. A. Brown covers the fundamental principles of gene cloning, polymerase chain reaction (PCR), genomic sequencing, and their diverse applications across biotechnology, medicine, and forensics.
The textbook is traditionally structured into three core sections, with specific updates tailored to the eighth edition:
1. Core Principles of Gene Cloning and DNA Analysis
  • Introduction to Gene Cloning: Why gene cloning and PCR are important in modern biology.
  • Vectors for Gene Cloning: Detailed exploration of cloning vehicles, including bacterial plasmids and bacteriophages.
  • Purification of DNA: How to isolate total cell DNA, plasmid DNA, and bacteriophage DNA.
  • Enzymes in Genetic Engineering: The use of restriction endonucleases, DNA ligases, and other polymerases to manipulate DNA.
  • Introduction of DNA into Living Cells: Transformation, transfection, and selection methods to identify recombinant colonies.
  • Polymerase Chain Reaction (PCR): Principles, methodologies, and new expanded material focusing on Real-time PCR (qPCR).
2. Research Applications (Sequencing and Gene Editing)
  • DNA Sequencing & Next-Generation Sequencing (NGS): Entirely rewritten chapters exploring modern platforms, transcriptomes, and the computational strategies used by researchers to assemble whole genome sequences.
  • Gene Editing: New and highly extended coverage of modern precision genome modification strategies, particularly CRISPR/Cas systems.
  • Studying Gene Expression & Function: How cloned genes are localized, transcribed, and translated into functional proteins within host systems.
3. Practical Applications in Biotechnology, Medicine, and Society
  • Production of Protein Biologics: Utilizing expression vectors for large-scale manufacturing of medical therapeutics, such as human insulin.
  • Medical Applications: Detailed coverage of molecular diagnostics, gene therapy, and updated material on the typing of human disease mutations.
  • Forensics and Archaeology: The role of DNA analysis in criminology, alongside fascinating case studies in paleogenomics, including the mapping of interbreeding between Neanderthals and Homo sapiens.
  • Agricultural Applications: Genetic modification of plants and animals for crop improvement and disease resistance.

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Topics

9781119640677 9781119640783 Agricultural Applications: Genetic modification of plants and animals for crop improvement and disease resistance Core Principles of Gene Cloning and DNA Analysis DNA Sequencing & Next-Generation Sequencing (NGS): platforms transcriptomes Enzymes in Genetic Engineering: restriction endonucleases DNA ligases and other polymerases to manipulate DNA Forensics and Archaeology: The role of DNA analysis in criminology Gene Editing: modern precision genome modification strategies particularly CRISPR/Cas systems Introduction of DNA into Living Cells: Transformation transfection and selection methods to identify recombinant colonies mapping of interbreeding between Neanderthals and Homo sapiens material on the typing of human disease mutations Medical Applications: molecular diagnostics gene therapy Polymerase Chain Reaction (PCR): Principles methodologies and new expanded material focusing on Real-time PCR (qPCR) Practical Applications in Biotechnology Medicine and Society Production of Protein Biologics: Utilizing expression vectors for large-scale manufacturing of medical therapeutics Purification of DNA: How to isolate total cell DNA plasmid DNA and bacteriophage DNA Research Applications (Sequencing and Gene Editing) Studying Gene Expression & Function: How cloned genes are localized transcribed and translated into functional proteins the computational strategies used by researchers to assemble whole genome sequences Vectors for Gene Cloning: cloning vehicles including bacterial plasmids and bacteriophages Why gene cloning and PCR are important in modern biology
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