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An Introduction to Genetic Engineering 4th Edition By Desmond S. T. Nicholl

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Pages: 470
File size: 35.22 MB
Format: Digital PDF
Course: Education
Category: eBook[PDF]

About this ebook

The fourth edition of An Introduction to Genetic Engineering by Desmond S. T. Nicholl covers the fundamental concepts, methodologies, and real-world applications of gene manipulation. The textbook is structurally divided into four core parts: [1, 2]

Part 1: Genetic Engineering in Context

Chapter 1: Introduction – The basic premise and scope of genetic engineering.
Chapter 2: The Story of DNA (New to this edition) – Historical context and the discovery journey of the genetic blueprint.
Chapter 3: Brave New World or Genetic Nightmare? – Explores bioethics, public perception, and societal impacts. [1, 2]

Part 2: The Basis of Genetic Engineering

Chapter 4: Introducing Molecular Biology – Core cell biology, transcription, and translation foundations.
Chapter 5: The Tools of the Trade – The primary enzymes, chemical toolkits, and components used to isolate and modify DNA.
Chapter 6: Working with Nucleic Acids – Basic technical handling, extraction, and quantification of DNA/RNA. [1, 2]

Part 3: The Methodology of Gene Manipulation

Chapter 7: Host Cells and Vectors – Plasmids, viruses, and cellular environments used to replicate foreign genes.
Chapter 8: Cloning Strategies – Step-by-step methods for creating recombinant DNA objects.
Chapter 9: The Polymerase Chain Reaction – Dedicated strategies and types of PCR amplification.
Chapter 10: Selection, Screening and Analysis of Recombinants – Identifying successful genetic transformations.
Chapter 11: Bioinformatics – Computational tools, algorithms, and biological data processing.
Chapter 12: Genome Editing (New to this edition) – Modern CRISPR/Cas9 systems and targeted gene modifications. [1]

Part 4: Genetic Engineering in Action

Chapter 13: Investigating Genes, Genomes and 'Otheromes' – DNA sequencing technologies, structural/functional genomics, and proteomics.
Chapter 14: Genetic Engineering and Biotechnology – Industrial scalings, protein production, and commercial biotechnology.
Chapter 15: Medical and Forensic Applications of Gene Manipulation – Gene therapies, diagnostic tools, and DNA profiling.
Chapter 16: Transgenic Plants and Animals – Agricultural modifications and the impact of GMOs.
Chapter 17: The Other Sort of Cloning – Somatic cell nuclear transfer and organism-level cloning concepts. [1, 2]