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Biomembranomics Structure, Techniques, and Applications 1st by Hongda Wang

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Pages: 536
File size: 9.62 MB
Format: Digital PDF
Course: Pharmaceutical Analysis
Category: eBook[PDF]

About this ebook

The book Biomembranomics: Structure, Techniques, and Applications (1st Edition) by Hongda Wang introduces the concept of applying "omics" systems biology specifically to biological membranes. It presents an updated compilation of the biophysical, chemical, and computational tools used to study plasma and organelle membranes at a single-molecule level. [1, 2]

The text covers the following specific topics and chapters:

1. Conceptual Framework & Baseline Structural Overview

Biomembranomics (New Concept): Introduction to the structure, properties, and core functions of cellular and organelle membranes, officially proposing the "biomembranomics" paradigm. [1, 2]
Mass Spectrometry: Outlines this ensemble technology to map out, identify, and quantify the overarching compositions of membrane proteins, phospholipids, and glycans. [1]

2. High-Resolution & Optical Imaging Techniques

High-Resolution Atomic Force Microscopy (AFM): Structural mapping and topographical resolution of cell membranes.
Super-Resolution Imaging: Highly localized mapping of specific membrane proteins and saccharides.
Fluorescence Microscopy: Targeted approaches for analyzing both the outer plasma membrane and complex intracellular membranes.
Single-Molecule Fluorescence: Investigating membrane protein dynamics, trafficking, and direct molecular interactions. [1]

3. Cryo-Electron & Vibrational Spectroscopy

Cryo-Transmission Electron Microscopy (Cryo-TEM): High-resolution structural determination of native cell membranes.
Cryo-Scanning Electron Microscopy (Cryo-SEM): Visualizing outer surfaces and structural domains of plasma membranes.
Infrared & Raman Spectroscopy: Non-destructive vibrational methods to monitor the chemical environment of the plasma membrane. [1]

4. Force Spectroscopy & Computational Applications

Single-Molecule Force Spectroscopy (SMFS): Applied specifically to look at cell membrane receptor dynamics, mechanical forces, and signal transduction cascades. [1]
AFM-Based Membrane Dynamics: Utilizing force measurement baselines to track physical variations, flexibility, and lateral organization on real-time membrane surfaces. [1]
Computer Simulations: Applying molecular dynamics (MD) simulations and computational models to track molecular transport, lipid arrangement, and membrane organization at timescales difficult to isolate via benchtop tools. [1]